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Thentamil, A.
- Hard Tissue Architecture of Tooth – No Strain with Stain
Authors
1 Department of Oral Biology, Penang International Dental College, NH-47, Sankari Main Road, Veerapandi Post, Salem - 636308, Tamil Nadu, IN
2 Department of Oral Biology, Penang International Dental College, NH-47, Sankari Main Road, Veerapandi Post, Salem-636308, Tamil Nadu, IN
3 Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, NH-47, Sankari Main Road, Ariyanoor, Salem - 636308, Tamil Nadu, IN
Source
Journal of Academy of Dental Education, Vol 3, No 1 (2017), Pagination: 5-9Abstract
One of the methods for visualizing and studying the hard tissues of the tooth is by preparation of ground sections of the desired area of the tooth. This method is relatively simple and enables us to appreciate the site and arrangement of various anatomical units of enamel, dentin and cementum. However, these sections are unstained and the features observed are based on the degree of light permeating through the sections. In this experiment, natural tooth were preserved in haematoxylin and eosin to study histological components of tooth. Twenty recently extracted natural teeth were utilized for this experiment and divided into two groups. In group 1, apical portion of the tooth were sealed and perforations were made on the different aspects of the tooth. In group 2, the apical portions of the ischolar_main were flattened and the apex kept open. Both groups were preserved in haematoxylin and eosin to permit imbibition of the stain. Following which, ground sections were prepared and viewed under a microscope.
Keywords
Haematoxylin and Eosin Stains, Histological Components, Ground Section.References
- Nanci A. Ten Cate’s oral histology. 8th ed. Elsevier Publications. 2013; p. 155–9. PMid:24421827 PMCid: PMC3852453
- Avery JK. Oral development and histology. 3rd ed. Thieme Publications. 2002; p. 163–5.
- Kumar GS. Orban’s Textbook of Oral Histology. 12th ed. Elsevier publications; Mosby. 2010. p. 95.
- Singh A, Gorea RK, Urveshisingla. Few tips for making ground sections of tooth for research purpose: A review. Journal of Punjab Academy of Forensic Medicine and Toxicology. 2006; 6:11–3.
- Williamson RA. Histological preparation of teeth and tooth growth. A review. Archives of Oral and Dental Research. 2015; 2:1–5.
- Aquaporins in Salivary Gland-The Water Fa(u)cet of an Acini?
Authors
1 Penang International Dental College, NH-47, Sankari Main Road, Veerapandi Post, Salem – 636308, Tamil Nadu, IN
2 Vinayaka Mission's Sankarachariyar Dental College, NH-47, Sankari Main Road, Ariyanoor, Salem – 636308, Tamil Nadu, IN
Source
Journal of Academy of Dental Education, Vol 4, No 1 (2018), Pagination: 12-16Abstract
Salivary glands are exocrine glands secreting saliva into the oral cavity. The primary function of the saliva is to protect and hydrate the mucosal structures of the oral cavity. The lubrication and hydration of the oral mucosa is provided by the water content of the saliva which forms approximately 99% of its composition. Aquaporins are water channels expressed in acini of salivary glands and play an important role in formation of saliva. Aquaporins are transmembrane water permeable proteins involved in transcellular water flow. In addition to being permeable to water, some Aquaporins can be permeable to small solutes, including cations, glycerol and gases. The present article reviews the basic histology of salivary gland, its ductal system and also physiology of secretion of saliva and highlights the role of Aquaporins in saliva formation.Keywords
Aquaporins, Clinical Application, Expression, Localization, Salivary Gland, Structure.References
- Nanci A. Ten Cate's oral histology. 8th ed. Elsevier Publications; 2013. p. 262–5.
- Kumar GS. Orban's textbook of oral histology. 14th ed. Elsevier Publications, Mosby; 2015. p. 247–250.
- Melvin JE. Chloride channels and salivary gland function. Crit Rev Oral Biol Med. 1999; 10(2):199–209. https://doi.org/10.1177/10454411990100020601 PMid:10759422
- Delporte C, Steinfeld S. Distribution and roles of Aquaporins in salivary glands: A review. Biochimicaet Biophysica Acta. 2006; 1758:1061–70. https://doi.org/10.1016/j.bbamem.2006.01.022 PMid:16537077
- Gresz V, Kwon TH, Hurley PT, Varga G, Zelles T, Nielsen S, Case RM, Steward C. Identification and localization of aquaporins water channels in human salivary glands. Am J Physiol Gastrointest Liver Physiol. 2001; 281:G247-G257. https://doi.org/10.1152/ajpgi.2001.281.1.G247 PMid:11408278
- Catalán MA, Nakamoto T, Melvin JE. The salivary gland fluid secretion mechanism: Mini-review. The Journal of Medical Investigation. 2009; 56:192–6. https://doi.org/10.2152/jmi.56.192 PMid:20224180
- Delporte C. Role of aquaporins in saliva secretion: A review. OA Biochemistry. 2013; 1(2):1-6. https://doi.org/10.13172/2052-9651-1-2-991
- Delporte C, Bryla A, Perret J. Aquaporins in salivary gland: From basic research to clinical applications: A review. International Journal of Molecular Science. 2016; 17(2):1– 13. https://doi.org/10.3390/ijms17020166 PMid:26828482 PMCid:PMC4783900
- Effects of Coconut and Olive Oil on Tooth and Restoration – In Vitro Study- Myth(oil)ogy?
Authors
1 Department of Oral Biology, Penang International Dental College, NH-47, Sankari Main Road, Veerapandi Post, Salem – 636308, IN
2 Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, NH-47, Sankari Main Road, Ariyanoor, Salem – 636308, Tamil Nadu, IN
Source
Journal of Academy of Dental Education, Vol 4, No 1 (2018), Pagination: 22-26Abstract
One of the natural and traditional methods adopted for obtaining whiter teeth is oil pulling. This is a simple and cost efficient method which has been used for decades and advised for overall maintenance of oral hygiene. However, there have been contradicting results regarding the efficacy of oil pulling on tooth whitening in previous studies. In this study, natural teeth (premolar teeth) were collected and divided into four groups and preserved in different mediums for 24 hours for a period of seven days to study the effects on hard tissue enamel. The teeth were observed every 24 hours and the difference in tooth whitening was compared using a tooth shade guide and evaluated for observable changes in colour.Keywords
Acrylic Tooth, Coconut Oil, Lime Juice, Olive Oil, Premolars, Shade Guide, Whitening Paste.References
- Naseem M, Khiyani MF, Nauman H, Zafar MS, Shah AH, Khalil HS. Oil pulling and importance of traditional medicine in oral health maintenance. International Journal of Health Sciences. 2017; 11(4):65–70.
- Gbinigie O, Onakpoya I, Spencer E, McCall MacBain M, Heneghan C. Effect of oil pulling in promoting oro dental hygiene: A systematic review of randomized clinical trials. Complementary Therapies in Medicine. 2016; 26:47–54. https://doi.org/10.1016/j.ctim.2016.02.011 PMid:27261981
- Singh A, Purohit B. Tooth brushing, oil pulling and tissue regeneration: A review of holistic approaches to oral health. Journal of Ayurveda and Integrative Medicine. 2011; 2(2):64–8. https://doi.org/10.4103/0975-9476.82525 PMid:21760690 PMCid:PMC3131773
- Wheater M, Friedl Z. Effect of oil pulling on tooth whitening in vitro. Journal of Advanced Oral Research. 2016; 7(1):20–3. https://doi.org/10.1177/2229411220160104
- Nam S-H, Kwun H-S, Cheon S-H, Kim H-Y. Effects of whitening toothpaste on color change and mineral contents of dental hard tissues. Biomedical Research. 2017; 28(9):3832–6.